Esther MF Lucas1,2,
Yuri Machado1,
Alexandre A Ferreira1,
Luiza MP Dolabella1,
Jacqueline A Takahashi1 
For correspondence:- Jacqueline Takahashi Email: jat@qui.ufmg.br Tel:+553134095754
Received: 1 October 2009 Accepted: 8 May 2010 Published: 25 August 2010
Citation: Lucas EM, Machado Y, Ferreira AA, Dolabella LM, Takahashi JA. Improved Production of Pharmacologically-active Sclerotiorin by Penicillium sclerotiorum. Trop J Pharm Res 2010; 9(4):365-371 doi: 10.4314/tjpr.v9i4.6
© 2010 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: The aim of this work was to study the optimum conditions for the production of sclerotiorin, a highly active secondary metabolite of Penicillium sclerotiorum under different cultural media.
Methods: A Brazilian strain of P. sclerotiorum was grown under different culture conditions in two liquid media (malt and a dextrose-peptone salt medium supplemented with sodium chloride) and in solid state fermentation in rice. Sclerotiorin production was monitored by high performance liquid chromatography (HPLC).
Results: Quantitative analysis of sclerotiorin content by HPLC indicated that sclerotiorin production reached the highest level (up to 313 + 10 mg.L-1) in the dextrose-based medium after 10 days of fermentation. Rice and malt broth showed lower production levels.
Conclusion: Enhanced production of P. sclerotiorin for pharmaceutical development can be achieved using dextrose-based cultures.
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